Abstract

Using fluorescent probe to study the binding reaction between Streptomycin sulfate and Bovine serum albumin

Author(s): Ying Guo, Baosheng Liu, Zhiyun Li, Lihui Zhang, Yukai Lu

After reaction of Bovine serum albumin and Streptomycin sulfate, the fluorescence intensity of Bovine serum albumin has no obvious change, so there is no way to directly research the binding reaction of Streptomycin sulfate and Bovine serum albumin by fluorescence spectrometry. In this paper, firstly, we study the interaction between EosinYand Bovine serum albumin using the method of fluorescence spectrumand ultraviolet visible absorption spectrometry. After adding the Streptomycin sulfate to the Bovine serum albumin and Eosin Y system, the fluorescence intensity of Bovine serumalbumin has recovered. This shows that the specific binding reaction of Streptomycin sulfate and Bovine serumalbumin has happened, and its reaction mechanism has been discussed by fluorescence probe EosinY.