Abstract

Ultrasonic extraction method for alizarin from roots of Morinda citrifolia

Author(s): Watsaka Siriangkhawut, Ing-on Kaewboo

An ultrasonic method for the extraction of alizarin from roots of Morinda citrifolia was investigated. The influence of four extraction variables on extraction efficiencies of alizarin was evaluated and compared with maceration. The optimal extraction conditions found were 0.1 g dried plant sample with soaked for 3 h in 15 mL of 60% aqueous ethanol and then extraction for 15min under ultrasonic irradiation in ultrasonic bath. Under the optimumconditions, the extraction yield of alizarinwas 14.80  0.02mg/ g with 95 - 102 % recovery. The reproducibility of the extraction method were in the range of 2.1 -2.5%for intra-day and inter-day extraction.