Abstract

The effect of SOCS3 on the epithelial stromal transformation of human hepatoma MHCC97-H cells

Author(s): Li-Le Wang

To study the effect of suppressor of cytokine signaling 3 (SOCS3) on the epithelial mesenchymal transition (EMT) of human hepatoma cell line MHCC97-H. Methods: human hepatocarcinoma cell line MHCC97-H was cultured in vitro. The cells were transiently transfected with 25 nmol / L SOCS3 siRNA (positive transfection group) and transfected with empty plasmid (negative control group). After 24 hours, the fluorescent expression of the transfected cells was observed by fluorescence microscope. 48 hours later, the morphological changes of the cells were observed by inverted microscope. The expression of E-cadherin, an epithelial marker of EMT, and a-SMA, an interstitial marker in MHCC97-H cells were detected by immunofluorescence staining. Results: compared with the negative control group, MHCC97-H cells successfully transfected with SOCS3 siRNA showed green fluorescence. After transient transfection of SOCS3 siRNA, the hepatoma cells changed from cobblestone shape with epithelioid characteristics to spindle and spindle shape with interstitial cell morphology. The expression of E-cadherin and a-SMA was detected by immunofluorescence. The results showed that the expression of e-eadherin, the epithelial marker of SOCS3 siRNA positive transfection group, decreased significantly, while the expression of a-SMA, the interstitial marker of cells, increased significantly. Conclusion: This study shows that the down-regulation of SOCS3 expression in hepatoma cells can promote the proliferation of hepatoma cells by changing their EMT phenotype, molecular and phenotypic characteristics, suggesting that SOCS3 may play an important role in the EMT of hepatoma cells. Regulating the expression level of SOCS3 can inhibit the occurrence and development of liver cancer, and provide a new idea for clinical prevention and treatment of liver cancer.