Abstract

The dosage of polyphenols in Elaeagnus angustifolia L. fruit extracts

Author(s): Leila Hambaba, Khamssa Boudjellal, Mohamed Cherif Aberkane, Mohamed Abdeddaim, Naoual Messaadia

A large number of ornamental and medicinal trees and shrubs possess very interesting biological properties finding applications in diverse domains including in food, medecine and pharmacy. In the present work, we have chosen the medicinal Elaeagnus angustifolia because it is the less frequently used in our country because of the ignorence of its nutritional and medical values. We are interested in the dosage and the study of the composition in secondary metabolites in different fruits extracts of Elaeagnus angustifolia including flavonoids, tannins and alkaloids. Phytochemical essays done on Elaeagnus angustifolia fruit extracts revealed the presence of flavonoids, tannins and the complete absence of alkaloids. Quantitaive analysis on TLC of the different extracts revealed the presence of a unique type of of flavonoids which is the rutin. The quantitaive analysis showed that the methanolic extract is rich with total flavonoids with a tenor of 3,94 ± 0,07 mg E AG/g of extract followed by dichloromethane and aqueous extractswith the value of 2,9 ± 0,7 mg E AG/g of axtract and 2,32 ± 0,48 mg EAG/g of extract. Flavonoids and tannins spectrophotometry dosage showed the presence of these substances in the polar extracts and their absence in the apolar extracts.