Abstract

Taxonomy and Study of Antimicrobial Activity of Two Halophilic Actinomycetes AH1 and AH2 Isolated from a Saline Soil of Bejaia (Algeria)

Author(s): Messis Abdelaziz, Bettache Azzeddine, Kecha Mouloud and Benallaoua Said

In this study we are interested in the taxonomic characterization of two isolates of halophilic actinomycetes, the identification of their antimicrobial and antifungal activities, and the determination of the chemical nature of bioactive molecules. Both strains AH1 and AH2 were isolated from saline soil in the region of Bejaia (Algeria). Based on morphological and chemotaxonomic characteristics, these isolates AH1 and AH2 are related to the genus Streptomyces. The growth and the antibiotic production by the two isolates are evaluated on two liquid culture media (ISP2 and M2) at different concentrations of NaCl. The results obtained showed that the antibiotic activity (antibacterial and antifungal) is very important. Extraction of antibiotic from Crude extracts by dichloromethane and n-butanol from the culture filtrate of the isolate AH1 and AH2 respectively, showed good activities, also, the crude extracts with hexane for both isolates showed a very important antifungal activity. The antibiotic complexes were located by bioautgraphy, and suggest that these molecules belong to subfamilies of aminoglycosides and polyether, and these antibiotics are non-polyenic.