Abstract

Study of partially purification AST activity in sera of Iraqi patients with diabetic nephropathy

Author(s): LaylaO.Farhan

Diabetic nephropathy is one of themajor causes of chronic renal failure.After many years of diabetes the delicate filtering system in the kidney becomes gradually destroyed.Initially becoming leaky to larger blood proteins such as albumin which are then lost in urine. Both serum urea and creatinine are widely used to assess the function of kidney.The aim of the study is to determinethe effect of diabetic nephropathy on serumAST level, and purification of enzyme in Diabetic Nephropathy and control.Laboratory investigations including fasting blood glucose, serum AST, ALT, Total protein, serum urea, uric acid, and creatinine had been measured in patients with Diabetic Nephropathy and control. In addition purification of AST from serumpatients withDiabetic Nephropathy and control by using ammonium sulphate (64-72 g)/ 100ml and gel filtration (Sephacryl S-300).Blood samples were obtained fromthe patients age [mean±SD: (49.00 5.20)] and the control [mean±SD :(39.20±5.38) ]age matched normal healthy individuals who came to the hospital for health checkup.fasting blood glucose,AST, Serum urea, and creatinine, significant difference between the patients and control group.ASTwith he highest specific activity afterPurificationchromatography on Sephacryl S-300.