Abstract

Spectrophotometric methods for simultaneous estimation of ramipril and valsartan in combined tablet dosage form

Author(s): Mrinalini C.Damle, Shradhanjali M.Singh, Amol B.Khetre, Rahul S.Darekar

Two simple, accurate and precise spectrophotometric methods for the simultaneous estimation of Valsartan (VAL) and Ramipril (RAMI) in combined dosage form have been developed and validated. First spectrophotometric method employed “Simultaneous equation method” using 210.0 nm and 249.0 nmas two wavelengths for absorbancemeasurement usingmethanol and 0.1N HCl as the solvents. Beer’s lawis obeyed in the concentration range of 1.25-40g/mL and 5-40g/mL for RAMI and VAL, respectively. Second method employed “Absorbance Correction Method”. The Wavelength selectedwas 274.5 nm.Molar absorptivities, Limit ofDetection (LOD) and Limit of Quantitation (LOQ) were calculated. Both methods have been successfully applied for the analysis of these drugs in a pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.