Abstract

Spectrophotometric determination of some fluoroquinolones antibacterial drugs in pure formand in pharmaceuticals formulations

Author(s): Ayman Abou El Fetouh Gouda, Ragaa El-Sheikh, Alaa Sayed Amin

A simple and sensitive spectrophotometric method has been developed for the determination of gatifloxacin (GT) andmoxifloxacin (MX) in pure form and in pharmaceutical formulations. The proposed method was based upon the formation of a ternary complex between palladium(II), eosin and the studied drugs in the presence of methyl cellulose as a surfactant and acetate buffer of pH (4.0-4.5). The ternary complex showed an absorption maxima at 552 and 549 nm for GT and MX, respectively. Apparent molar absorptivitieswere 2.4975104 and 3.663104Lmol-1cm-1 and Sandell’s sensitivities were 0.015 and 0.011g cm-2 for GT and MX, respectively. The solution of ternary complex obeyed Beer’s law over the concentration range of 2.0-10 and 1.0-8.0 g ml-1 for GT and MX, respectively with minimum detection limits 0.216 and 0.184 g ml-1 for GT and MX, respectively. The proposed method was successfully applied to the analysis of the studied drugs in their pharmaceutical formulations. The results obtained were in good agreement with those obtained using the reported method. A proposal of the reaction pathway was presented.