All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Spectrofluorimetric Determination of Tramadol in Formulation and Biological Fluids

Author(s): A. Anton Smith, R. Manavalan, K. Kannan and N. Rajendiran

A simple and reliable spectrofluorimetric method has been developed for the determination of tramadol HCl in the pharmaceutical preparation and biological fluids. The fluorescence of the product was found to have excitation at max 272 nm and emission emi at 298 nm. The method shows high sensitivity with linearity range from 0.07 to 0.4 5g/mL. The lower limit of detection (LOD) was found to be 1.127 x 10-3 5g/mL, 4.202 x 10-3 5g/mL and limit of quantification (LOQ) was found to be 3.415 x 10-3 5g/mL, 1.273 x 10-2 5g/mL in formulation and biological fluids, respectively. The different experimental parameters affecting the fluorescence intensity were carefully studied and optimized. The proposed method was applied successfully for determination of tramadol in the pharmaceutical preparation and biological fluids. The percentage recovery was found to be 99.477 ± 0.73 and 102.48 ± 1.433 for formulation and biological fluids, respectively.


Share this       
Google Scholar citation report
Citations : 9398

International Journal of Chemical Sciences received 9398 citations as per Google Scholar report

Indexed In

  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • Cosmos IF
  • Geneva Foundation for Medical Education and Research
  • ICMJE

View More