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Abstract

Simultaneous Estimation of Metronidazole and Ciprofloxacin by RP-HPLC Method in Bulk Drug and Suspension

Author(s): A. B. Patel, N. J. Shah and N. M. Patel

A simple, accurate and precise reverse phase high performance liquid chromatographic method has been developed and validated for the estimation of metronidazole and ciprofloxacin simultaneous determination in combined dosage forms. A Lichrospher 100 RP-180, C18 column was used as stationary phase and mobile phase contain phosphate buffer : acetonitrile (80 : 20, v/v) and final pH adjusted to 5.45 ± 0.02 with 10 % v/v o-phosphoric acid. Measurements were made at the effluent flow rate of 1.0 mL/min with injection volume 20 :L and ultraviolet (UV) detection at 290 nm, as both components show reasonable good response at this wavelength. The retention times of metronidazole and ciprofloxacin were 3.51 min and 6.84 min, respectively. The method was validated in terms of linearity, accuracy, precision, robustness and specificity. Linearity of metronidazole and ciprofloxacin was in the range of 1-70 :g/mL for both, respectively. Average percentage recoveries obtained for metronidazole and ciprofloxacin were 100.03 % and 99.13 %, respectively. The limit of detection and limit of quantification were found to be 0.5 and 1.0 μg/mL for metronidazole, respectively and for ciprofloxacin were 0.4 and 1.0 μg/mL, respectively. The method is useful in the quality control of bulk manufacturing and pharmaceutical dosage forms.


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International Journal of Chemical Sciences received 9398 citations as per Google Scholar report

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  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • Cosmos IF
  • Geneva Foundation for Medical Education and Research
  • ICMJE

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