Abstract
Simultaneous determination of cetrizine and ambroxol in formulation by RP-HPLC
Author(s): Lakshmi Sivasubramanian, V.NiraimathiA simple, precise and rapid reversed phase HPLC method was developed for the simultaneous estimation of cetrizine(CT) and ambroxol(AM) in combined formulation. Themethodwas carried out on aHypersilBDS C18 column using a mixture ofwater:methanol (pH adjusted to 3.5 with orthophosphoric acid): tetrahydrofuran(60:37:3 v/v/v) and detection was carried out at 230 nm. The drug CT showed linearity in the range of 4-32mcg/ ml and AM in the range of 48-384mcg/ml. Limits of quantification was found to be 20 and 4ng/ml for CT andAM respectively.
