Abstract

Sensitive Spectrophotometric Methods For The Determination Selenium By Using Leuco Methylene Blue Reagent In Environmental And Cosmetic Samples

Author(s): P.Chiranjeevi, P.Reddy Prasad, D.Rekha, J.Dilip Kumar, B.Krishnapriya, P.Subramanyam

Simple, sensitive, selective and rapid spectrophotometric method is developed for the determination of selenium (IV) in biological samples. This method is based on the oxidation reaction of leuco methylene blue (LMB) reagent with selenium (IV) to form a blue color derivative at pH-4.0. Which is having maximum absorption peak value at 605 nm. The reaction is instantaneous and color product is stable for 7 days at room temperature. Beer’s law is obeyed in the concentration range of 0.4 - 4.2 μg ml-1 for selenium (IV). Molar absorvity and sandell’s sensitivity of the system is found to be 3.64 × 105 ml-1 cm-1, 0.0004 μg cm-2 respectively. The detailed study of the various interfering ions confirmed the high selectivity of the proposed method. The accuracy of the proposed method is expressed in terms of t-test and variance ratio of ftest. The results obtained were good argument with the reported method at the 95% confidence level. The proposed method is successfully applied for the determination of Se (IV) in environmental and cosmetic samples.