Abstract

Second-derivative UV spectrometric determination of ganciclovir in its solid dosage form

Author(s): Hemant K.Khairnar, Shradha R.Jedge, Brijesh D.Patel, Sandip S.Kshirsagar, Rajesh J.Oswal

Ganciclovir is an acyclic guanosine analogue used in the treatment of cytomegalovirus (CMV) infection andAIDS in humans. During the development of Ganciclovir solid dosage form, formulation compositionswere constantly varied. A fast and reliable method for the dissolution and release testing of Ganciclovir was highly desirable to support formulation screening. A second derivative UV spectroscopic method was developed for determination of Ganciclovir in the solid dosage form.After carefully choosing a zero-crossing technique of second derivative UV measurement at 253 nm, the selectivity and sensitivity of Ganciclovir was comparable to the previously developed HPLC method. In comparison with the direct UV method, second derivative UV spectroscopy eliminates the interference fromUV absorbing excipients, which often results in a standard error of 2– 10%. This method is also fast and economical in comparison to the more time-consuming HPLC method regularly used for formulation screening. Finally, this method has been validated to be precise and accurate, and is demonstrated to be an excellent alternative to HPLC method for the dissolution and release testing of Ganciclovir in the solid dosage form.