Abstract

RAPD marker as an ideal tool for evolutionary genetics for the analysis of Aeromonas hydrophila

Author(s): Agniswar Sarkar, Mousumi Saha, Pranab Roy

Aeromonas hydrophila isolates fromfish, meats (chicken,Mutton) and water fromriver were characterized through RandomlyAmplified Polimorphic DNA(RAPD) analysis. PCR amplification of DNAproduced different identical amplicons and given some unique bands by using RAPD primers (OPA1 to OPA10) on agarose gel electrophoresis and showed genetic heterogeneity within the species. Polymorphism of RAPD profile was evident with a unique pattern and indicating its usefulness as an ideal tool for evolutionary genetics because of morphological and biochemical characterization sometimes gives contradictory results.