Abstract

Quantitative analysis of diosgenin in costus speciosus extracts by HPTLC with ultraviolet absorption densitometry

Author(s): J.Y.Nehete, M.S.Bhatia

Diosgenin is one of the phytoconstituent present in Costus speciosus (Koen) J.E. Sm. This plant and its extracts are used for treatment of fever, snake bites, jaundice, and as a purgative, astringent and antibacterial. In the present study an attempt has been made to develop a HPTLC method for quantitative estimation of diosgenin in fractions of methanol extract. This HPTLC method was found to be reproducible, accurate and precise and could detect and quantitate nanogram level concentrations of diosgenin. The developed HPTLCmethod would be an important tool in the evaluation of diosgenin content in polyherbal formulations.