Abstract

Purification and characterization of natural food biopreservative produced fromBacillus subtilis R1 isolated froma refreshing drinkwhey

Author(s): Nivedita Sharma, Riti Kapoor, Neha Gautam, Bhanu Neopaney

Bacteriocin is a safe and natural food preservative produced frombacteria. Hyper bacteriocin producing bacterial strain R1 isolated from fermented milk drink-whey has been identified as Bacillus subtilis by 16S rRNAgene technique. Bacteriocin produced fromB.subtilis R1 showed strong inhibition against many of the food borne serious pathogens. Bacteriocin produced from B.subtilis R1 was purified by gel exclusion chromatography. Molecular weight of bacteriocinwas found to be 13KDa. Purified bacteriocinwithstood temperature up to 121oC, found active atwider pH range and was sensitive to trypsin. This antibacterial substance showed bactericidal effect against its sensitive indicators which are main food borne / spoilage causing microorganisms.