Abstract

Proteomics and its analysis

Author(s): Tim Jason

A change in the content of the cerebrospinal fluid (CSF), which is released by a number of distinct central nervous system (CNS) structures, will properly represent pathological events. Proteomics provides a thorough system-level perspective for analyzing CSF proteins. In this work, a number of analytical techniques, including sample preparation, two-dimensional liquid and gel electrophoresis, mass spectrometry, bioinformatics, and non-gel approaches are reviewed. In the postgenomic age, it is still very difficult to measure the characteristics of endogenous cell proteins on a full proteome level, including expression level, subcellular localization, and turnover rates. The levels of relevant proteins cannot be predicted quantitatively, and there is little or no information on other protein characteristics.