Abstract

Protective role of selenium-enriched yeast against hepatic necrosis induced by thioacetamide in experimental animals

Author(s): Hanaa H.Ahmed, El-S.M.E.Mahdy, Hatem A.El-Mezayen, Rehab M.Hussien

This study aimed at evaluating the protective role of Se-Y against TAA induced hepatic necrosis in rats. Sixty-four adult female rats were divided into 8 groups : (1) served as control, (2) was administered TAA, (3 and 4) were given two dose levels of Se (0.95 and 0.47 mg kg-1 day-1) respectively with simultaneous administration of TAA, (5 and 6) were given two dose levels of yeast (66.5 and 33.25mg kg-1 day-1) respectivelywith simultaneous administration of TAA and (7 and 8) were given two dose levels of Se-Y (66.5 and 33.25mg kg-1 day-1) respectivelywith simultaneous administration of TAA. SerumAST,ALT,ALP activities, hepatic SOD, CAT, GPx , eNOS activities, MDA and NO as well as eNOS activity. Histopathological examination of liver tissue sections was carried out. The results revealed that TAAintoxication caused significant increase in serumALT,AST,ALP activities, hepatic MDA, NO levels and eNOS activity, while it produced significant decrease in hepatic SOD, CAT and GPx activities versus the control. Histopathological investigation of liver tissue sections of TAAintoxicated rats showed necrosis with inflammatory cell infiltration. Cotreatment with Se, yeast or Se-Y produced significant protection against TAA-induced hepatic necrosis as indicated bymarked improvement in the studied biochemical markers and histopathological feature of liver tissue. In conclusion, Se, yeast and Se-Y could protect the rat liver from TAAinduced hepatic necrosis with special reference to Se-Y which showed the most pronounced effect.