Abstract

New Spectrophotometric Method for the Determination of Metoclopramide Hydrochloride in Pharmaceutical Preparations based on Coupling with Doxycycline Hyclate

Author(s): Abbas Noor Alshirifi and Marowa H. Abbas

A new simple, rapid, sensitive, selective, and accurate method for the spectrophotometric determination of metoclopramide hydrochloride (MCP-HCl) in different pharmacetical preparations has been developed. Metoclopramide hydrochloride is widely used in the treatment nausea and vomiting. The spectrophotometric method is based on diazotization of primary amine group of (MCP-HCl) with sodium nitrite and hydrchloric acid followed by reaction with other drug doxycycline hyclate (DOX-HYC) as coupling agent in alkaline medium to form a stable yellow azo dye, showed amaximum absorption at 452 nm. Beers law was obyed in the concentration range of 0.1-10 μg.mL-1 with amolar absorptivity (3.81×104) L.mol-1.cm-1, and sandell’s sensitivity 0.009 μg.cm-2, respectively. The analytical parameters were optimized as the fallowing: The best temperature is 1-30°C), the best time to complete reaction is 15 min and the best volume of doxycycline hyclate (DOX-HYC) solution is 1.5 mL. Limit of detection (LOD), and limit of quantification (LOQ) are 0.012 ppm, and 0.043 ppm, respectively, the recoveries range 98.58%-100.61%. The method was successfully applied to the analysis of the (MCP-HCl) in its pharmaceutical preparations (tablets, syrup, injection and orals drop).