Abstract

High quality genomic DNA from Lens culinaris without liquid nitrogen

Author(s): Kripi Vohra, Ashwani Kumar, Vivek Kumar Gupta, Gagandeep Pal

Lens culinaris Medikus also known as Lens esculenta Moench., lentil or masur is polyphenols, saponins and carbohydrates rich food grain. The polysaccharides and polyphenols interfere in (deoxyribonucleic acid)DNA extraction and isolation which is an important and basic step for molecular, biological and genetic study of a plant. The present study was an attempt to isolate high quality genomic DNA fromLens culinarisMedikus leaves usingmodified Cetyl trimethyl ammoniumbromide (CTAB)method without using liquid nitrogen. The genomic DNAwas isolated fromleaves using absolute ethanol and chloroform ethanol solution as fixatives and was quantified using spectroscopic and agarose gel electrophoresis method. The concentration of DNA extracted from the leaves of the Lens culinaris Medikus using absolute ethanol and chloroform ethanol solution as fixativeswas found to be in the range of 2.08- 2.30 µg/ml. The DNA isolated using absolute ethanol was better in quality as it showed better optical density. DNA was observed as blue band on agarose gel. This method is rapid, simple and efficient for isolating DNA fromplants rich in phenolic compounds without the use of liquid nitrogen.