Abstract

Effect of Indigofera Tinctoria Linn Against Isoproterenol Induced Myocardial Infarction on Albino Wistar Rats

Author(s): M. Nagasaraswathi, P. Rafi Khan, M. A. Aleemuddin, K. Gopi Chand and K. Sravani

The present study was undertaken to evaluate the effect of hydroalcoholic leaf extract of Indigofera tinctoria linn (ITE) against isoproterenol (ISO) induced myocardial infarction in albino wistar rats. Rats were pre-treated with ITE (100 mg/Kg & 200 mg/Kg) daily for 28 days. After pre-treatment, Isoproterenol (5.25 mg/Kg and 8.50 mg/Kg, S.C) was injected to rats at an interval of 24 hrs for two consecutive days to induce myocardial infarction. Cardio protective activity was investigated by estimating the levels of Transaminases (Aspartate Transaminase, Alanine Transaminase), Lactate Dehydrogenase (LDH), Creatine Phosphokainase (CPK), Cardiac total protein and antioxidant enzymes such as Superoxide Dismutase (SOD), Reduced Glutathione (GSH), Glutathione Peroxidase (GPx), and Thiobarbituric acid reactive substances (TBARS) in both serum and heart tissue. The levels of serum marker enzymes Transaminases (aspartate transaminase, alanine transaminase), LDH, CPK were significantly (p < 0.05) increased and cardiac total protein was decreased in isoproterenol treated group. ITE pre treated groups showed decrease in the levels of serum marker enzymes by increase in cardiac total protein. Antioxidant levels such as SOD, GSH and GPx was decreased significantly in serum of ISO treated group with increase in TBARS levels. ITE pre treated groups showed significantly (p < 0.05) increased antioxidant levels such as SOD, GSH and GPx with decreased TBARS levels. The histopathological evidence indicates that ISO alters all the bio-chemical parameters in the body and it can be protected by ITE treatment. This study concluded that hydroalcoholic extract of Indigofera tinctoria linn possess protection to ameliorate the myocardial infarction induced by isoproterenol in albino wistar rats.