Abstract

Effect of Azadirachta excelsa leaves extract on uterine histology and testosterone level in alloxan induced diabetic rats.

Author(s): S.Nurdiana, M.S.Nur Zulaikha, M.I.Syamimi Wahida, J.Elizabeth Jega, S.Nurul Ã?Â?Ã?Â?Ã?Â?Ã?Â?Izzati

The protective effect of Azadirachta excelsa leaves extract against infertility inmale and female diabetic rats was studied. The fertility experiment was done on twenty four Sprague Dawley male and female rats respectively. Themale and female ratswere divided into four groups each which one of them was kept as normal control, while the others were rendered diabetic by intraperitoneal injection of alloxan (130mg/kg) for 28 consecutive days. On day 29 of treatment, after 8 hours of fast, blood were withdrawn for blood glucose and testosterone analysis, uterine were harvested for histological study and semen samples were collected to determine the characters of epididymal sperm.Administration of A.excelsa was significantly reduced the level of blood glucose in bothmale and female diabetic rats. Examination on semen was found that the plant extract was increased the number of sperm for 37.32% while the sperm cell abnormalities was revealed to be decreased on the end of experimental period. Microscopic observation was showed that A.excelsa able to reduce atrophy and increase the proliferation of stromal cells in the uterine of female diabetic rats. These results suggest that A.excelsa can potentially be developed as an alternative agent to restore the characters of epididymal sperm and to normalize distribution of stromal cells is an indicator towards improving the fertility of men and women with diabetes.