Abstract

Different stabilityÃ?Â?Ã?Â?Ã?Â?Ã?Â?indicating techniques for the determination of triclabendazole

Author(s): Nesrin K.Ramadan, Afaf O.Mohamed, Sara E.Shawky, Maissa Y.Salem

Three simple, accurate and sensitive methods were developed for the determination of triclabendazole (TCZ) in presence of its degradation product. Method (A) was depending on third derivative spectrophotometry 3D, then measuring the peak amplitude at 266 nm. Method (B) was a TLC method, using silica gel 60 F254 plates; the optimized mobile phase was ethyl acetate/ methanol/ ammonium hydroxide (8:1:0.2 by volume). The spots were scanned densitometrically at 300 nm.Method (C) was an HPLC method, performed on C18 column using acetonitrile/ 0.05M potassium dihydrogen phosphate (60:40 v/v), the pH was adjusted to 3.5±0.2 with ortho-phosphoric acid as a mobile phase with a flow rate of 1.5 ml/min. Detection was performed at 300 nm. Linearity ranges were 5 – 40 µg/ml for method (A), 0.5 – 5 µg/band for method (B) and 0.5 – 5 µg/ml for method (C), the mean percentage recoveries were 100.0 ± 1.0%, 100.5 ±1.2% and 99.8 ±1.1% for methods (A), (B) and (C) respectively. The proposed methods were found to be specific for triclabendazole in presence of up to 70% of its degradation product for method (A) and 90% of its degradation product for methods (B) and (C). Statistical comparison between the results obtained by these methods and the manufacturerÂ’s method was done, and no significance difference was obtained.