Abstract

Development and validation of UPLC method for emtricitabine, tenofovir and efavirenz in pharmaceutical prepartion

Author(s): Pravish Tiwari, Ravi Yadav, Avinash K., V.Vaidya, P.A.Sathe, Deepali Gangrade

A simple, sensitive and validated UPLC method has been developed to determine Emtricitabine, Tenofovir and Efavirenz simultaneously in synthetic mixture form. Chromatographic separation was achieved on a BEH Phenyl column using a mixture of buffer pH 6.5,Methanol andAcetonitrile in the ratio of 45:27.5:27.5 (v/v) at a wavelength of 260nm. Linearity of the method was found to be in the concentration range of 0.026-0.079g/ml for Emtricitabine and 0.024-0.088g/ml forTenofovir and 0.08-0.24g/mlEfavirenz with correlation coefficient greater than 0.999. The total eluting time for the three components is less than 1.5 minutes. The method can be used for simultaneous determination of Emtricitabine, Tenofovir and Efavirenz.