Abstract
Development And Validation Of RP-HPLC Method For The Estimation Of Torasemide In Pure Form And In Pharmaceutical Dosage Forms
Author(s): D.Gowri Sankar, M.Vamsi Krishna, N.Sujatha, L.A.Rama Prasad, B.Sneha LathaA simple Reverse phase HPLC method was developed for the estimation of torasemide in bulk and in pharmaceutical dosage forms. Torasemide was chromatographed on a reversed phase C18 column in isocratic mode with a mobile phase comprising of Methanol and Water (pH adjusted to 2.5 with ortho phosphoric acid) in the ratio of ( 75:25 v/v). The mobile phase was pumped at a flow rate of 1ml/min and the eluents were monitored at 214nm. The calibration curve was linear in the range of 0.1 - 50μg/ml. The proposed method was found to be simple, precise and hence can be applied for routine quality control analysis of torasemide in bulk drugs and in pharmaceutical dosage forms.
