Abstract

Development and validation of HPLC method for determination of Oxcarbazepine in bulk and pharmaceutical formulation

Author(s): K.Sivarami Reddy, N.V.S.Naidu

A rapid, reliable, sensitive, selective, precise and accurate High Performance Liquid Chromatography method for analysis of Oxcarbazepine in pharmaceutical formulations. Separation was achieved with a reversedphaseWaters (alliance) HPLC C18, 150X 4.6, 5µ, employing UV detection at215nm. The solvent systemconsisted of mixed buffer andAcetonitrile in the ratios (55: 45 v/v) at a flowrate of 1.0ml/min. The run timewas 10.0min and the retention time was 3.871min. The linear regression data for the calibration plots showed good linear relationship with r2=0.999 in the concentration range of 7.5-45g/ml. The developed method was validated for accuracy, precision, robustness, detection and quantification limits as per the ICH guidelines. The wide linearity range, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better for the quantification of Oxcarbazepine