Abstract
Development and validation of anHPLCmethod for the analysis of nateglinide in pharmaceutical dosage forms
Author(s): K.Srinivasa Rao, K.SrinivasA simple reverse phase HPLC method was developed for the determination of nateglinide present in pharmaceutical dosage forms. A Hypersil ODS C18, 4.6mmïÂÂ250mm, 5µm column with mobile phase acetonitrile: phosphate buffer pH-5.5,70:30%v/v was used. The flow rate was 1.0 ml/ min and effluent was monitored at 210 nm. Ezetemibe is used as internal standard. The retention times were 4.47min and 3.308min for nateglinide and ezetimibe respectively. The linearity range was found to be 0.5-200µg/ ml for nateglinide. The proposed method was also validated.
