Abstract
Development and validation of an HPLC method for the analysis of repaglinide in pharmaceutical dosage forms
Author(s): K.Srinivasa Rao, S.Satyanarayana Raju, K.SrinivasA simple reverse phase HPLC method was developed for the determination of repaglinide present in pharmaceutical dosage forms. A Hypersil ODS C18, 4.6mm250mm, 5mcolumn fromSupelco (India), withmobile phase methanol: ammonium acetate buffer (pH-4) (80:20) was used. The flowratewas 1.0ml/min and effluentwasmonitored at 240 nm. Ritonavir is used as internal standard. The retention times were 6.19 min and 5.23 min for repaglinide and ritonavir respectively. The linearity range was found to be 0.5-200ïÂÂg/ml for repaglinide. The proposed method was also validated.
