Determination of ketoprofen enantiomers in plasma and exudate by liquid chromatography-Mass spectrometry

VÃƒÂƒÃ‚ÂƒÃƒÂ‚Ã‚Â­ctor HormazÃƒÂƒÃ‚ÂƒÃƒÂ‚Ã‚Â¡bal; ÃƒÂƒÃ‚ÂƒÃƒÂ‚Ã‚Â˜yvin ÃƒÂƒÃ‚ÂƒÃƒÂ‚Ã‚Â˜stensvik

Analytical Chemistry: An Indian Journal
ISSN (PRINT): 0974-7419

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Abstract

Determination of ketoprofen enantiomers in plasma and exudate by liquid chromatography-Mass spectrometry

Author(s): V�?�?�?�?�?�?�?ctor Hormaz�?�?�?�?�?�?�?¡bal, �?�?�?�?�?�?�?�?yvin �?�?�?�?�?�?�?�?stensvik

A liquid chromatographic-mass spectrometrymethod for the determination of ketoprofen enantiomers in swine/cat plasma and exudate is described. Plasma /exudate proteins were precipitated with acetone-trichloroacetic acid and centrifuged. The supernatant (~ 500uL) was filtered through a centrifuge filter tube before it was diluted and injected into the LC-MS/ MS. The recovery for both R and S ketoprofen varied from 97 to 98% in plasma and exudate. The limits of quantification of R and S were 10 ng/mL and the limit of detection was 5 ng/mL for both R and S in plasma and exudate, based on 0.5 mL swine plasma or exudate. ïÃ‚ÂƒÃ‚Â“ 2013 Trade Science Inc. - INDIA.