Abstract
Determination of betahistine hydrochloride using a sensitive fluorescent probe
Author(s): Jun Yang, Li-Ming Du, Guang-Quan Wang, Hao Wu, Xu Jing, Wen-Xian Liu, Yun-Long FuThe inclusion interaction of cucurbit[7]uril (CB[7]) with Betahistine Hydrochloride (BH) was studied using fluorescent probe titration method. Significant quenching of the fluorescence intensity of the CB[7]-palmatine (PAL) fluorescent probe was observed when BH was added, showing that BH was accommodated into the hydrophobic cavities of CB[7]. The hydrophobic interaction between CB[7] and BHmainly contributed to the formation of 1:1 type CB[7]-BH.The association constants of the complexes formed between the host and the guest were determined. The competing reaction and the supramolecular interaction mechanisms between the BH and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry, 1H NMR, and molecular modeling calculations.
