Abstract

Derivative spectroscopy: development and validation of new colorimetric method for the estimation ofmetadoxine in bulk and solid dosage form

Author(s): Pradeep Kumar, Naresh Chandra Joshi, Anuj Malik, Niranjan Kaushik, Ashok Kushnoor, Nagaraj Gowda

Chemicallymetadoxine is pyridoxol L-2-pyrrolidone-5-carboxylate an ion pair that combines pyridoxine and Pyrrolidone carboxylate[1].Metadoxine exerts several actions that are beneficial to patients with alcoholic liver diseases[2]. It increase the clearance of alcohol and acetaldehyde and decreases the damaging effect of free radicals, restores ATP and glutathione levels, reduces steatosis and prevents liver fibrosis[3].After thorough survey of literature it is found that Metadoxine has been estimated by HPLC[4] and HPTLC[5] methods, but no spectrophotometric methods are cited in the literature, we report simple and sensitive colorimetric method in visible region for the estimation of Metadoxine in pure and pharmaceutical dosage forms by derivative spectroscopy.