Abstract

Chromatography: Techniques of Separation

Author(s): Jacob Taylor

Chromatography is a significant biophysical technique for qualitative and quantitative analysis that allows for the separation, identification, and purification of the components of a mixture. Size and shape, total charge, hydrophobic groups on the surface, and binding capacity with the stationary phase are all factors that can be used to purify proteins. Ion exchange, surface adsorption, partition, and size exclusion are four separation approaches based on molecule properties and interaction type. Column, thin layer, and paper chromatography are examples of chromatography procedures that use a stationary bed. One of the most prevalent methods of protein purification is column chromatography