Abstract

Chemically Modified Carbon Paste Electrode For Potentiometric Analysis Of Cyproheptadine Hydrochloride In Serum And Urine

Author(s): Hazem M.Abu-Shawish, Salman M.Saadeh

A new carbon-paste electrode for cyproheptadine hydrochloride (CyCl) was prepared and fully characterized in terms of composition, response time, thermal stability and usable pH ranges. The electrode active recognition is by liquid ion-exchange mechanism via the use of the cyproheptadinium-tetraphenylborate (CY-TPB) as ion-exchanger dissolved in tricresyl phosphate (TCP) as a more suitable solvent mediator for the paste and sodium tetraphenylborate (Na-TPB) as additive. The modified electrode showed a Nernstian slope of 58.5+2 mV/decade over the concentration range of 5.2×10-6-1.0x10-2 M with very low detection limit of 4.2×10-7. The electrode exhibits good selectivity for CyCl with respect to a large number of inorganic cations, sugars, amino acids. The developed electrode was applied to the potentiometric determination of cyproheptadinium ion in its aqueous solution, pharmaceutical preparations and biological fluids (serum and urine). The proposed potentiometric methods offer the advantages of simplicity, accuracy, automation feasibility and applicability to turbid and colored sample solutions.