Abstract

AValidated chiral liquid chromatographicmethod for the enantiomeric separation of �?�?�?¯�?�?�?�?�?�?¢-amino-�?�?�?¯�?�?�?�?�?�?¢-(4-bromophenyl) propionic acid

Author(s): P.Madhavan, B.M.Rao, B.Pravin, T.Thilakkumar, K.Arun, T.Sravan, K.Sivakumar, K.B.Chandrasekhar

A chiral liquid chromatographic method was developed for the enantiomeric separation of -amino--(4-bromophenyl) propionic acid using donor- acceptor (Pirkle) column. The enantiomers of -amino--(4- bromophenyl) propionic acidwere resolved on a 2504.6mm(R,R)Whelk- 01 column, with 5m particle size, which was accompanied with a 1 cm long guard column. The mobile phase used for the separation was nhexane, ethanol, tri fluoro acetic acid, iso propyl amine(95:05:0.1:0.025).The resolution between the enantiomers was found >2.5. The presence of iso propyl amine in the mobile phase was played an important role in enhancing chromatographic efficiency and the resolution between the enantiomers. The developed method was extensively validated and proved to be robust. The limit of detection(LOD) for(R) and(S)--amino--(4- bromophenyl) propionic acid enantiomers are 0.3 and 0.3g/ml respectively. The limit of quantification (LOQ) for (R) and (S)--amino--(4- bromophenyl) propionic acid enantiomers are 1 and 1g/ml respectively.