Abstract

Analysis Of Bovine-Derived Demineralized Bone Extracts

Author(s): R.Murugan, S.Ramakrishna, K.Panduranga Rao

Demineralized bone factors are capable of stimulating bone regeneration through an osteoinductive mechanism and thus it has been recognized as a good bone graft. In this study, a kind of demineralized bone extracts (DBX) derived from bovine tibia by a chemical route. The extracts thus obtained were analyzed for their bio-chemical and physical properties using various techniques and results provided quite interesting insights into the demineralization process. There is no significant evidence of mineral phase associated with the connective tissue detected during chemical as well as physical testing, indicating the formation of DBX. The spectroscopic and thermal analyses indicated the demineralization process at a reasonable limit as they do not show any characteristic sign of inorganic elements associated with bone minerals. The particle size and distribution of processed DBX lay within the optimal level required for osteoinduction. This kind of bone extracts may used as a bone graft material and as a substrate for the growth of biomimetic apatites.