Abstract

An ultra fast and sensitive detection of 165 drugs of abuse in human urine using polarity switching ultra performance liquid chromatography tandemmass spectrometry

Author(s): Sachin Dubey, ShobhaAhi,Alka Beotra, TejinderKaur, Shila Jain

The screening of wide variety of prohibited substances in a time bound manner by adhering to latest World Anti-Doping Agency (WADA) guidelines is a challenging task for doping control laboratories. The revised criterion of detection limits (WADATD2013MRPL) has further required the doping laboratories to reviewtheir testing procedures. The presentwork was aimed at developing a fast, sensitive and robust analytical method based on solid phase cleanup (SPE) and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to achieve the required detection levels. The method development involved optimization of deconjugation of phase II metabolites, SPE usingmixed-mode ion cartridges for extraction of analytes of wider chemistries; and fast polarity switching UPLC-MS/MS detection. The developed method was validated to detect approximately 165 compounds and/or metabolites prohibited by WADA. The eight minutes runtime allowed testing of approximately 180 samples in 24 hours at the limit of detection (LOD) of 50%belowrequired detection levels.