Abstract

A Stability Indicating Assay Method For Propranolol Tablets By High Performance Liquid Chromatography For Stability Studies

Author(s): Monika Jain, S.N.Shrivastava

A simple and stability indicating HPLC assay procedure had been developed and validated for propranolol tablets stability samples. The mobile phase consisted of buffer(5.0 g Triethylamine /1000ml water pH 4.0 by HCOOH): acetonitrile:in the ratio of (70:30) isocratic elution is carried out under ambient condition at flow rate of 1.5 ml min- 1 and detector was set at 225 nm. The column selected was Luna, C18, 5µm packing, 4.6 mm x 250 mm and injection volume was 20 µl. The procedure separated propranolol and potential degradation product. The retention time of propranolol is 8.5 min and asymmetry was 1.55. The instrument precision obtained was 0.19 %.The procedure provided a linear response in the range of 50 – 150 % of target concentration (r=1.000). Forced degradation study shows, response of main drug is reduced in acid, alkali and peroxide degradation. The method was validated for accuracy, robustness and solution stability was obtained up to 27 hrs.