Abstract
A RP-HPLC Method for Estimation of Tramadol HCl and Chlorzoxazone in Tablet Formulation-Validation
Author(s): Dutta M and Wakode SA simple, rapid and selective method involving reverse phase liquid chromatography is developed for the concurrent determination of Tramadol HCl and Chlorzoxazone in tablet formulation. The chromatographic parameters involved using a Promosil C18 column with dimensions 4.6*250 mm, 5 μ and Acetonitrile and 0.1% Trifluroacetic acid in water in the ratio 60:40 (v/v) as the mobile phase. The flow rate set was of 1.0 mL/min and detected at 275 nm using UV detector. The linearity of Tramadol HCl and Chlorzoxazone were in the range of 25.00 μg/mL-45.00 μg/mL and 125.00 μg/mL-225.00 μg/mL respectively. Standard addition method was used to calculate percentage recovery. The average amount of recovery was found to be 98.8% and 99.7% for Tramadol HCl and Chlorzoxazone respectively. The method was found to be accurate and precise for determining both the drugs simultaneously.
