Abstract

A rapid densitometric method for simultaneous quantification of two biologically active compounds in Vernonia cinerea whole plant powder using HPTLC

Author(s): Willy Shah, Suhas Pednekar, Sunita Shailajan, Vikas Vaidya

In present study, an attempt has been made to develop a simultaneous HPTLCmethod of quantitative determination of -Sitosterol and Lupeol in Vernonia cinerea whole plant powder. Chromatography was performed on silica gel 60 F254 HPTLCplate,with toluene:methanol, 88:12 (v/v), asmobile phase. After development, plates were treated with Lieberman-Burchard Reagent, detection and quantification were performed by densitometry at 366 nm in fluorescence mode. The method was validated in terms of its linearity, limits of detection and quantification and precision following standard protocols. -Sitosterol and Lupeol was found to be linear in the range of 15.0g mL-1-35.0g mL-1 and 45.0g mL-1 - 105.0g mL-1 respectively. Average content of -Sitosterol and Lupeol in whole plant powder of Vernonia cinerea was found to be 0.48960.00035mg and 1.4882  0.0002mg respectively. The method was found to be simple, precise, accurate, specific and sensitive and can be used for routine quality control of herbal raw materials and for the quantification of these compounds in plant materials.