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Abstract

A Novel Spectrofluorimetric Method for the Determination of Gemifloxacin in Bulk and Pharmaceutical Formulation

Author(s): P. Nagaraju, P. Ravisankar, G. Devala Rao and Y. Rajendra Prasad

Novel selective, rapid, simple, sensitive, econom ic and reproducible spectrofluorimetric method was developed for the determination of Gemifloxacin in bulk as well as pharmaceutical formulations. Gemifloxacin, as a primary aromatic amine, reacts fluorescamine, which is highly sensitive fluorogenic reagent used in many investigations. This method was based on the reaction between Gemifloxacin and fluorescamine in borate buffer solution of pH 8.5 to give a highly fluorescent derivative. This reaction product was measured spectrofluorimetrically at 483 nm after excitation at 353 nm under optimum conditions, linear relationship with best correlation co efficient 0.9999 and the linearity was detected in between the range of 100-1000 ng mL -1 . The average % recovery obtained was quantitatively as 100.37%. The intraday and interday precision was found to be 0.074 % and 0.096%, respectively. The limit of detection was found to be 0.123 μg/mL and the limit of quantification was 0.369 μg/mL. Therefore, this newly recommended spectrofluorimetric method is most suitable for estimation of Gemifloxacin and observed to be validated for calculation of accuracy, precision, robustness, LOD and LOQ. So this method becomes highly congenial for analys is of Gemifloxacin in tablet form . Therefore, spectrofluorimetric method is considered as most convenient analytical technical method for the drug determination in pharmaceutical preparations, quality co ntrol and clinical laboratories


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Indexed In

  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • Cosmos IF
  • Geneva Foundation for Medical Education and Research
  • ICMJE

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