All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

A fluorescence spectroscopic study of the reaction mechanism of Tylosin Tartrate with Lysozyme

Author(s): Gaixia Li, Baosheng Liu, Rong Han, Ying Guo

Under simulated physiological conditions, the interaction between Tylosin Tartrate (TT) and Lysozyme (LYSO) was investigated at pH=7.40 by fluorescence spectroscopy. The results indicated that TT could strongly quench the intrinsic fluorescence of LYSO. By determining the quenching constants of the reaction between TT and LYSO at different temperatures, the quenching mechanism was proven to be a static quenching process. The thermodynamic parameters (ΔH, ΔS) of the reaction between TT and LYSO were obtained by the Van 't Hoff equation, and were 27.80 KJ mol-1 and 166.28 J mol-1 K-1, respectively. The results showed that hydrophobic interaction between TT and LSYO was dominant. Synchronous fluorescence spectra revealed that the conformation of LYSO was changed. This method could be applied to measure the content of TT.


Share this       
Awards Nomination

Table of Contents

Google Scholar citation report
Citations : 875

BioTechnology: An Indian Journal received 875 citations as per Google Scholar report

Indexed In

  • CASS
  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Cosmos IF
  • Directory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs
  • Euro Pub
  • ICMJE

View More

Flyer